关键词: 一氧化氮供体型药物, 牙龈卟啉单胞菌, 抗菌作用, 种植体周围炎, 活性氧, 钙离子

Abstract: Objective  To study the effect of nitric oxide （NO） on the in vitro antibacterial activity of Porphyromonas gingivalis (P.g). Methods  The antibacterial performance of different concentrations of NO (10,30,50 mmol/L) on P.g was assessed by colony forming units,total NO detection kit,reactive oxygen species detection kit,and calcium ion fluorescent probe Fluo-4 AM. Moreover,the effects of NO treatment on intracellular levels of reactive oxygen species （ROS） and calcium ions in P.g cells were also examined.  Results  NO exhibited significant in vitro antibacterial activity against in P.g cells,and its antibacterial activity was positively correlated with the concentration of NO drugs and treatment duration (P<0.05). NO treatment significantly increased intracellular levels of ROS and calcium ions in P.g cells (P<0.05). Conclusion  NO can effectively inhibit the activity and growth ability of P.g,and cause intracellular ROS and calcium ion metabolism disorders. Our study demonstrates the potential therapeutic effect and clinical application of NO donor drugs in peri-implantitis.

Key words: nitric oxide donor drugs, Porphyromonas gingivalis (P.g), antimicrobial activity, peri-implantitis, reactive oxygen species, Ca²⁺